Even when in a static position, data acquired from 6 Degrees of Freedom (DoF) trackers is affected by noise, which is typically called jitter. In this study, we analyzed the effects of 3D rotational jitter on Virtual Reality (VR) controllers in a 3D Fitts' law experiment, which explored how such jitter affects user performance. Eight subjects performed a Fitts' law experiment with or without additional jitter on the cursor. Results show that while error rate significantly increased above ±0.5° jitter and subjects' effective throughput started to decrease significantly above ±1° jitter, there was no significant effect on users' movement time. Further, the Fitts's law movement time model was affected when ±2° jitter was applied to the tracker. According to these results, ±0.5° jitter on the controller does not significantly affect user performance for the tasks explored here. The results of our study can guide the design of 3D controller and tracking systems for 3D user interfaces.